De volgende ploegen hebben hun deelname aan de Midden-Brabant Poort Omloop 2024 bevestigd / The following teams have confirmed their participation in the MBPO for 2024
| Team | Nationality | Category |
|---|---|---|
| BEAT CYCLING CLUB | Netherlands | UCI Continental Team |
| DIFTAR CONTINENTAL CYCLINGTEAM | Netherlands | UCI Continental Team |
| ISRAEL PREMIER TECH ACADEMY | Israel | UCI Continental Team |
| METEC - SOLARWATT P/B MANTEL | Netherlands | UCI Continental Team |
| NOVAPOR SPEEDBIKE TEAM | North Macedonia | UCI Continental Team |
| PARKHOTEL VALKENBURG | Netherlands | UCI Continental Team |
| TARTELETTO - ISOREX | Belgium | UCI Continental Team |
| DUTCH FOOD VALLEY CYCLING TEAM | Netherlands | Club Team |
| EMBARK TBW STUART HALL CYCLING | Great-Britain | Club Team |
| GIANT STORE ASSEN CT - NWVG | Netherlands | Club Team |
| GRC JAN VAN ARCKEL | Netherlands | Club Team |
| JEGG-DJR Academy | Netherlands | Club Team |
| MONDA VAKANTIEPARKEN - IJSSELSTREEK CT | Netherlands | Club Team |
| SENSA - KANJERS VOOR KANJERS CYCLING TEAM | Netherlands | Club Team |
| SN - VITAE BIM BAM COACHING RT | Great-Britain | Club Team |
| THRIVA-SRCT | Great-Britain | Club Team |
| WILLEBRORD WIL VOORUIT | Netherlands | Club Team |
| WILVO GROUP - TWC DE KEMPEN | Netherlands | Club Team |
| TEMPO-HOPPENBROUWERS | Netherlands | Club Team |
| VOLHARDING CYCLING TEAM | Netherlands | Club Team |
| RIDE UNITEDCYCLING TEAM | Netherlands | Club Team |
| AW GROEP - SWABO CYCLINGTEAM | Netherlands | Club Team |
| REGIOTEAM OOST | Netherlands | Mixed Team |